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Updated: Nov 19, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
The role of micropeptides in biology
Rui Vitorino1,2, Sofia Guedes3,4, Francisco Amado3,4
1Departamento de Cirurgia E Fisiologia, Faculdade de Medicina da Universidade Do Porto, UnIC, Porto, Portugal. rvitorino@ua.pt.
Abstract:
Micropeptides are small polypeptides coded by small open-reading frames. Progress in computational biology and the analyses of large-scale transcriptomes and proteomes have revealed that mammalian genomes produce a large number of transcripts encoding micropeptides. Many of these have been previously annotated as long noncoding RNAs. The role of micropeptides in cellular homeostasis maintenance has been demonstrated. This review discusses different types of micropeptides as well as methods to identify them, such as computational approaches, ribosome profiling, and mass spectrometry.
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