Related Experiment Video
Updated: Nov 19, 2025
Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
Published on: January 3, 2025
Plasma levels of sirtuin-1 in patients with cerebrovascular stroke
Imam M Esmayel1, Samia Hussein2, Ehab A Gohar1
1Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Insights
Plasma levels of sirtuin 1 (SIRT1) are lower in acute cerebrovascular stroke patients. Lower SIRT1 may indicate a higher risk for stroke, suggesting its potential as a predictive biomarker.
Area of Science:
- Biochemistry
- Neuroscience
- Cardiovascular Diseases
Background:
- Circulatory system diseases cause one-third of deaths in Egypt, with stroke ranking third.
- Stroke, encompassing ischemic and hemorrhagic types, is a significant health concern.
- Sirtuins (SIRTs) are NAD+-dependent deacetylases involved in physiological processes, activated by low cellular energy.
Purpose of the Study:
- To quantify plasma SIRT1 levels in acute cerebrovascular stroke.
- To evaluate SIRT1 as a potential biomarker for predicting stroke risk.
- To compare SIRT1 levels between ischemic and hemorrhagic stroke and correlate them with stroke severity.
Main Methods:
- A case-control study involving 108 participants (54 controls, 54 stroke patients).
- Stroke patients were divided into ischemic (28) and hemorrhagic (26) subgroups.
- Plasma SIRT1 levels were measured using enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Plasma SIRT1 levels were significantly lower in acute stroke patients compared to controls.
- No significant difference in SIRT1 levels was observed between ischemic and hemorrhagic stroke groups.
- SIRT1 levels positively correlated with hemoglobin and serum potassium, and negatively with triglycerides and stroke score.
Conclusions:
- Plasma SIRT1 levels are reduced in patients with acute cerebrovascular stroke.
- SIRT1 shows potential as a biomarker for predicting the risk of acute cerebrovascular stroke.
Background:
In Egypt, circulatory system diseases are responsible for one-third of the annual deaths. Stroke comes 3rd after heart diseases and liver diseases. Stroke includes two types: ischemic and hemorrhagic. The sirtuins (SIRTs) are a family of histone deacetylases that are nicotinamide adenine dinucleotide (NAD)+ dependent. They are activated under conditions of decreased cellular energy stores and are involved in the control of several physiological processes.
Objectives:
To measure the plasma levels of SIRT1 in acute cerebrovascular stroke, to assess its role as a possible biomarker in predicting the risk of acute cerebrovascular stroke, to compare its levels between the two groups of stroke patients, and to evaluate the association between its levels and the severity of stroke. Also, to assess the correlations between the plasma SIRT1 levels and the variables that might play a role in the severity of acute cerebrovascular stroke.
Methods:
This is a case-control study carried out on one hundred and eight participants. The participants were divided into two groups: group A (control group) included fifty-four individuals. Group B (acute cerebrovascular stroke group) included fifty-four stroke patients of two subgroups: B1: twenty-eight patients suffering from acute ischemic stroke and B2: twenty-six patients suffering from acute hemorrhagic stroke. Measurement of the plasma levels of SIRT1 was performed using the enzyme-linked immunosorbent assay (ELISA).
Results:
Regarding SIRT1 levels, acute stroke groups were significantly lower than the control group with no significant difference between ischemic and hemorrhagic groups. There were positive correlations between SIRT1 levels and each of the hemoglobin levels and serum potassium levels. There were negative correlations between SIRT1 levels and each of triglycerides (TG) and stroke score.
Conclusion:
Plasma levels of SIRT1 are lower in patients with acute cerebrovascular stroke than in control. Furthermore, SIRT1 may act as a possible biomarker for predicting the risk of acute cerebrovascular stroke.
More Related Videos
08:41Lateral Chronic Cranial Window Preparation Enables In Vivo Observation Following Distal Middle Cerebral Artery Occlusion in Mice
Published on: December 29, 2016
10:03Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
Related Concept Videos
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...