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Updated: Aug 9, 2026
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Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Published on: September 9, 2014
A macrophage-monocyte cell line from a dog with malignant histiocytosis
M L Wellman1, S Krakowka, R M Jacobs
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Abstract:
The DH82 cell line was established from the neoplastic progenitor cells of canine MH and was characterized as histiocytic in origin based on light microscopic and ultrastructural morphology, positive staining reactions for alpha naphthyl acetate esterase and acid phosphatase, presence of Fc receptors, phagocytosis of latex beads, and plastic adherence in culture.
