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Related Experiment Video

Updated: Nov 19, 2025

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Published on: May 16, 2019

Projection Microstereolithographic Microbial Bioprinting for Engineered Biofilms.

Karen Dubbin¹, Ziye Dong², Dan M Park²

¹Engineering Directorate, Lawrence Livermore National Laboratory, Livermore, California 94550, United States.

|January 28, 2021

Summary

This summary is machine-generated.

Researchers developed a 3D bioprinting technique to pattern microbes in hydrogels. This method enables the study of microbial biofilm structure and function, including metal ion absorption and sensing.

Keywords:

Additive Manufacturing Bacteria Biofilm Bioprinting Bioremediation Biosensor Microbial Printing Stereolithography

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Last Updated: Nov 19, 2025

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Published on: May 16, 2019

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Published on: November 18, 2016

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Area of Science:

Microbiology
Biotechnology
Biogeochemistry

Background:

Microbial communities are essential for ecosystems and biogeochemical cycles.
The role of microbial three-dimensional (3D) organization in function remains largely unexplored.

Purpose of the Study:

To develop a novel technique for patterning microbes in 3D geometries.
To investigate how biofilm structure influences microbial activity and function.
To explore applications in metal ion absorption and sensing using engineered biofilms.

Main Methods:

Developed a projection stereolithography technique for 3D bioprinting of microbes within hydrogel architectures.
Monitored bacterial biomass accumulation to confirm post-print cell viability.
Fabricated multi-strain fluorescent biofilms to demonstrate biological and geometric complexity.
Assessed metal ion sequestration and uranium sensing capabilities of patterned engineered *Caulobacter crescentus*.

Main Results:

Successfully demonstrated the viability of microbes post-3D bioprinting.
Achieved fabrication of complex, multi-strain fluorescent biofilms.
Showcased geometric effects on metal ion absorption efficiency and uranium sensing by engineered microbes.

Conclusions:

This study presents the first demonstration of stereolithographic printing of microbes.
The developed technique offers a powerful tool for creating engineered biofilms and complex 3D microbial cultures.
Opens new avenues for understanding structure-function relationships in microbial communities and for designing novel biotechnological applications.