CO₂-converting enzymes for sustainable biotechnology: from mechanisms to application

To realize a circular, carbon-neutral economy, it will become important to utilize the greenhouse gas CO₂ as a sustainable carbon source. Carboxylases, the enzymes that capture and convert gaseous CO₂ are the prime candidates to pave the way towards realizing this vision of a CO₂-based bio-economy. In the last couple of years, the interest in using and engineering carboxylases has been steadily growing. Here, we discuss how basic research on the mechanism of CO₂ binding and activation by carboxylases opened the way to develop new-to-nature CO₂-fixing enzymes that found application in the development of synthetic CO₂-fixation pathways and their further realization in vitro and in vivo. These pioneering efforts in the field pave the way to realize a diverse CO₂-fixation biochemistry that can find application in biocatalysis, biotechnology, and artificial photosynthesis.