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Nucleosome Remodeling

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...

The Nucleosome Core Particle

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...

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Related Experiment Video

Updated: Nov 19, 2025

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Published on: December 12, 2017

Resolving nucleosomal positioning and occupancy with MNase-seq.

Wei Hang Deng¹, Xin Hui Li¹

¹Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Yi Chuan = Hereditas

|January 29, 2021

Summary

This summary is machine-generated.

Micrococcal nuclease sequencing (MNase-seq) precisely maps nucleosome positioning and occupancy across genomes. This chromatin analysis method reveals insights into DNA transcription, replication, repair, and disease mechanisms.

Keywords:

chromatin remodeling chromatin structure micrococcal nuclease next-generation sequencing (NGS)nucleosome

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Area of Science:

Chromatin biology
Genomics
Molecular biology

Background:

Nucleosomes form the fundamental structure of chromatin.
Nucleosome positioning and occupancy are critical for DNA transcription, replication, and repair.
Understanding chromatin structure is key to deciphering genomic processes.

Purpose of the Study:

To review the technical principles of Micrococcal Nuclease sequencing (MNase-seq).
To highlight recent advances and future perspectives of MNase-seq in chromatin biology.
To emphasize the role of MNase-seq in uncovering disease mechanisms.

Main Methods:

Detailed explanation of MNase-seq technical principles.
Focus on resolving nucleosome positioning and occupancy.
Genome-wide analysis of nucleosomal landscapes.

Main Results:

MNase-seq is a highly effective method for genome-wide nucleosomal analysis.
Investigation of nucleosomal landscapes across various organisms.
Revealed commonalities and differences in chromatin organization.

Conclusions:

MNase-seq is essential for precise nucleosome mapping.
The method offers mechanistic insights into fundamental genomic processes.
Future applications of MNase-seq hold promise for understanding diseases.