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Detection of Human Immunodeficiency Virus Type 1 HIV-1 Antisense Protein ASP RNA Transcripts in Patients by Strand-Specific RT-PCR
Published on: November 27, 2019
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Antisense Transcripts and Antisense Protein: A New Perspective on Human Immunodeficiency Virus Type 1
Juliette Savoret1, Jean-Michel Mesnard1, Antoine Gross1
1Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Université de Montpellier, Montpellier, France.
Frontiers in Microbiology
|January 29, 2021
Summary
Human Immunodeficiency Virus type 1 (HIV-1) antisense transcripts and the AntiSense Protein (ASP) encoded by them are conserved in pandemic strains. Their roles in viral infection warrant further investigation.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- The Human Immunodeficiency Virus type 1 (HIV-1) genome was predicted to contain an Open Reading Frame (ORF) encoding an AntiSense Protein (ASP) in 1988.
- Subsequent research confirmed the presence of HIV-1 antisense transcripts and ASP in infected cells, alongside an immune response targeting ASP.
Purpose of the Study:
- To review recent findings on HIV-1 antisense transcripts and ASP.
- To discuss their potential functions in HIV-1 infection and roles in other viral infections.
- To suggest future research directions.
Main Methods:
- Literature review of studies on HIV-1 antisense transcripts and ASP.
- Analysis of the conservation of the *asp* gene within HIV-1 groups.
- Comparative discussion of antisense transcripts and ASPs in other viruses.
Main Results:
- The *asp* gene is exclusively conserved within the pandemic group M of HIV-1.
- Evidence supports the existence of HIV-1 antisense transcripts and ASP.
- An immune response targeting ASP has been observed.
Conclusions:
- HIV-1 antisense transcripts and ASP are significant viral components, particularly in pandemic strains.
- Further research into their functions in HIV-1 and other viral infections is warranted.
- The study of these elements may reveal novel therapeutic targets or insights into viral pathogenesis.
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