The effect of laryngeal nerve stimulation on phonation: a glottographic study using an in vivo canine model

The present investigation was designed to examine the effect of change in vocal fold mass and stiffness on vocal fold vibration. To do this, the effect of variation in superior laryngeal nerve stimulation (SLNS) and recurrent laryngeal nerve stimulation (RLNS) was studied Photoglottography (PGG), electroglottography (EGG), and subglottic pressure (Psub) were measured in seven mongrel dogs using an in vivo canine model of phonation. The PGG, EGG, and Psub signals were examined at three frequencies (100, 130, and 160 Hz) for SLNS and RLNS, using a constant rate of air flow. Increasing SLNS, which caused a contraction of the cricothyroid muscle, produced a marked increase in F0, little change in Psub, an increase in open quotient (OQ), and a decrease in the closed quotient (CQ) of the glottal cycle. Increasing RLNS, which caused activation of the intrinsic laryngeal muscles, produced a modest increase in F0, a marked increase in Psub, no change in the OQ, and an increase in CQ. Phase quotient (Qp), which describes the interval between opening of the lower and upper fold margins, decreased with increasing RLNS and did not change significantly with increasing SLNS. Based upon changes in F0, Psub, OQ, CQ, and Qp, SLNS provides a physiologic correlate of the tension parameter Q, and RLNS provides a physiologic correlate of the parameter Psub in the Ishizaka and Flanagan two-mass model.