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Related Concept Videos

Cell Migration01:09

Cell Migration

17.8K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

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Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
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Cell Motility through Blebbing01:16

Cell Motility through Blebbing

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Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
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Microtubules in Cell Motility01:24

Microtubules in Cell Motility

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Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

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Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
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Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Related Experiment Video

Updated: Nov 19, 2025

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
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A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

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Bioimage Analysis and Cell Motility.

Aleix Boquet-Pujadas1,2,3, Jean-Christophe Olivo-Marin1,2, Nancy Guillén1,4

  • 1Institut Pasteur, Bioimage Analysis Unit, 25 rue du Dr. Roux, Paris Cedex 15 75724, France.

Patterns (New York, N.Y.)
|January 29, 2021
PubMed
Summary
This summary is machine-generated.

Bioimage analysis (BIA) provides objective quantification for cell movement studies. Advanced BIA methods are crucial for uncovering biological insights in complex data, driving discovery in mechanobiology and microenvironment research.

Keywords:
Entamoeba Histolyticabioimage analysiscell biologycell biophysicscell morphologycell motilitycell segmentationcell trackingcomputational biologyinverse problemsmechano-biology

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Last Updated: Nov 19, 2025

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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Area of Science:

  • Cellular biology
  • Biophysics
  • Image analysis

Background:

  • Biological experiments historically relied on qualitative observations.
  • Classical image processing techniques enhanced objectivity and reproducibility in cell movement studies.
  • Bioimage analysis (BIA) has been integral to understanding cell motility.

Purpose of the Study:

  • To review classical and modern bioimage analysis techniques for studying cell movement.
  • To illustrate how quantification in BIA augments biological data and discovery.
  • To highlight the growing importance of BIA in mechanobiology and microenvironment research.

Main Methods:

  • Cell segmentation
  • Cell tracking
  • Morphology descriptor analysis
  • Ameboid motility as a case study

Main Results:

  • Quantification amplifies subtle differences in cell behavior.
  • Statistical analysis uncovers general laws of cell movement.
  • BIA integrates physical insights into biological data.
  • Quantitative imaging is essential for mechanobiology and microenvironment studies.

Conclusions:

  • Bioimage analysis is becoming a primary driver of biological discovery.
  • Human visual analysis is insufficient for increasingly complex biological data.
  • BIA offers non-invasive methods for biophysical measurements and physiological relevance.