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IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

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Corrole-Substituted Fluorescent Heme Proteins.

Christopher M Lemon^1,2,3, Michael A Marletta^1,2,4

¹Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, United States.

Inorganic Chemistry

|January 29, 2021

Summary

This summary is machine-generated.

Researchers developed novel fluorescent proteins by incorporating phosphorus corrole into stable heme proteins. This approach enhances fluorescence properties, offering a promising alternative to traditional fluorescent proteins for biological applications.

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Measurement of Heme Synthesis Levels in Mammalian Cells

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Area of Science:

Biophysical Chemistry
Protein Engineering
Fluorescent Probes

Background:

Traditional fluorescent proteins have optical limitations, including weak red/near-infrared emission and broad emission profiles.
Enhancing fluorescent protein photophysical properties requires novel cofactors integrated into stable protein scaffolds.

Purpose of the Study:

To incorporate a fluorescent phosphorus corrole into stable heme proteins (H-NOX and HasA).
To evaluate the optical properties of these novel corrole-protein conjugates.
To explore the potential of corrole-heme protein conjugates as improved fluorescent probes.

Main Methods:

Incorporation of a fluorescent phosphorus corrole into H-NOX and HasA heme proteins.
Steady-state and time-resolved optical spectroscopy to analyze photophysical properties.
Comparison of fluorescence characteristics with free corrole and traditional fluorescent proteins.

Main Results:

The HasA-corrole conjugate showed enhanced fluorescence compared to the free corrole.
The H-NOX-corrole conjugate exhibited quenched fluorescence.
Corrole-substituted proteins displayed more intense fluorescence with narrower spectral profiles than traditional fluorescent proteins in the same spectral window.

Conclusions:

Fluorescent corrole complexes can be successfully incorporated into heme proteins.
This study provides a foundation for developing novel fluorescent proteins with improved optical properties.
Corrole-heme protein conjugates offer a new avenue for bioimaging and sensing applications.