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Related Experiment Video

Updated: Nov 19, 2025

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
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Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators.

Akiko Fujishima1, Kazumasa Takahashi1, Mayumi Goto1

  • 1Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.

Plos One
|January 29, 2021
PubMed
Summary

This study visualizes intracellular sodium (Na+) and potassium (K+) concentrations during mouse embryonic development. Researchers observed dynamic electrolyte shifts, with Na+ decreasing and K+ increasing during blastocoel formation, offering new insights into early embryogenesis.

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Area of Science:

  • Developmental Biology
  • Cell Physiology
  • Biochemistry

Background:

  • Electrolytes like sodium (Na+) and potassium (K+) are crucial for embryonic development.
  • Previous studies used indirect methods (inhibitors, knockout mice) to assess electrolyte roles, lacking direct intracellular concentration data during embryogenesis.

Purpose of the Study:

  • To directly visualize and quantify intracellular Na+ and K+ concentrations throughout mouse embryonic development.
  • To investigate the dynamic changes in electrolyte levels during key developmental stages like morula, blastocyst, and hatching.
  • To assess Na+/K+ ATPase activity based on electrolyte concentration changes in response to ouabain.

Main Methods:

  • Utilized fluorescent electrolyte indicators CoroNa Green AM (for Na+) and ION Potassium Green-2 AM (for K+).
  • Directly imaged intracellular electrolyte concentrations in mouse embryos at morula, blastocyst, and hatching stages.
  • Assessed Na+/K+ ATPase activity by observing responses to ouabain, a specific inhibitor.

Main Results:

  • Observed dynamic changes in intracellular electrolyte concentrations during embryogenesis.
  • Found a decrease in intracellular Na+ and an increase in K+ during blastocoel formation.
  • Demonstrated differential Na+/K+ ATPase activity in trophectoderm cells based on their location relative to the blastocoel and inner cell mass.

Conclusions:

  • This study provides the first direct visualization of intracellular electrolytes during mouse embryonic development using novel indicators.
  • Observed electrolyte dynamics correlate with known Na+/K+ ATPase activity, validating the imaging method.
  • The methodology offers a powerful tool for detailed studies of cell physiology and embryonic development.