Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

144
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
144
Responses to Salt Stress02:02

Responses to Salt Stress

13.8K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.8K
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

187
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
187
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

248
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
248
Transcription01:10

Transcription

153.3K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
153.3K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

14.2K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated CO<sub>2</sub> alters soybean physiology and defense responses, and has disparate effects on susceptibility to diverse microbial pathogens.

The New phytologist·2025
Same author

Soybean genomics research community strategic plan: A vision for 2024-2028.

The plant genome·2024
Same author

Investigating the Role of Known Arabidopsis Iron Genes in a Stress Resilient Soybean Line.

International journal of molecular sciences·2024
Same author

The Soybean <i>Rpp3</i> Gene Encodes a TIR-NBS-LRR Protein that Confers Resistance to <i>Phakopsora pachyrhizi</i>.

Molecular plant-microbe interactions : MPMI·2024
Same author

Coupling VIGS with Short- and Long-Term Stress Exposure to Understand the Fiskeby III Iron Deficiency Stress Response.

International journal of molecular sciences·2023
Same author

Comparing Early Transcriptomic Responses of 18 Soybean (<i>Glycine max</i>) Genotypes to Iron Stress.

International journal of molecular sciences·2021

Related Experiment Video

Updated: Nov 19, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.6K

Gene Expression Responses to Sequential Nutrient Deficiency Stresses in Soybean.

Jamie A O'Rourke1, Michelle A Graham1

  • 1Corn Insects and Crop Genetics Research Unit, USDA-Agricultural Research Service, Ames, IA 50010, USA.

International Journal of Molecular Sciences
|January 30, 2021
PubMed
Summary

Soybean plants exposed to sequential iron and phosphate deficiency (-Fe-Pi) showed a unique gene expression pattern. This response involved genes typically related to reproduction, not stress, highlighting plant transcriptome plasticity.

Keywords:
IDCabiotic stressglycine maxiron stressphosphate stresstranscriptomics

More Related Videos

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

25.7K
Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
08:39

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

1.9K

Related Experiment Videos

Last Updated: Nov 19, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.6K
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

25.7K
Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
08:39

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

1.9K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Agricultural Science

Background:

  • Crops face repeated abiotic stresses during the growing season, impacting yield.
  • Understanding plant stress tolerance mechanisms is crucial for mitigating yield loss.
  • Most research focuses on single stress events, not realistic sequential exposures.

Purpose of the Study:

  • To investigate the transcriptional profile of soybean plants under sequential iron and phosphate deficiency (-Fe-Pi).
  • To identify conserved genes across different repeated stress conditions.
  • To understand the unique transcriptional responses to sequential stress exposure.

Main Methods:

  • Soybean plants were subjected to sequential iron and phosphate deficiency stress.
  • Transcriptional profiling was performed to analyze gene expression changes.
  • Gene expression data was compared with previous studies on repeated stress exposures.

Main Results:

  • A core set of genes was identified as conserved across repeated stress exposures (-Fe-Pi, -Fe-Fe, -Pi-Pi).
  • Sequential stress (-Fe-Pi) induced the expression of genes typically associated with plant reproduction.
  • These reproductive genes were not previously recognized as part of stress response pathways.

Conclusions:

  • Plant transcriptomes exhibit significant plasticity in response to sequential abiotic stresses.
  • Sequential stress responses can involve unexpected gene sets, such as those related to reproduction.
  • Further research is needed to fully elucidate the complexity of plant stress response pathways.