Related Experiment Video

Updated: Nov 19, 2025

An Orthotopic Murine Model of Human Prostate Cancer Metastasis
06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

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Long Noncoding RNA HOXA11-AS and Transcription Factor HOXB13 Modulate the Expression of Bone Metastasis-Related Genes

Aya Misawa1, Yukihiro Kondo2, Hiroyuki Takei3

  • 1Department of Molecular Medicine and Anatomy, Nippon Medical School, 1-1-5 Sendagi, Tokyo 113-8602, Japan.

Genes
|January 30, 2021
PubMed

Insights

Prostate cancer metastasis is promoted by the HOXA11-AS long noncoding RNA and HOXB13 transcription factor. They regulate key signaling pathways involved in bone metastasis, offering potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Long noncoding RNAs (lncRNAs) are critical regulators of gene expression with roles in cancer development.
  • Prostate cancer bone metastasis involves complex molecular signaling pathways.

Purpose of the Study:

  • To investigate the role of HOXA11-AS in prostate cancer bone metastasis.
  • To identify upstream regulators and downstream targets of HOXA11-AS in this context.

Main Methods:

  • Analysis of HOXA11-AS expression in prostate cancer bone metastasis cell lines.
  • Investigating the effects of HOXA11-AS on PC3 cell invasion and proliferation.
  • Identifying HOXB13 as an upstream regulator.
  • Studying the regulation of CCL2/CCR2, ITGAV, ITGB1, and IBSP signaling.
  • Assessing the effects of secreted HOXA11-AS on osteoblastic cells.

Main Results:

  • HOXA11-AS is highly expressed in prostate cancer bone metastasis cell lines and promotes PC3 cell invasion and proliferation.
  • HOXB13 acts as an upstream regulator of HOXA11-AS.
  • The HOXB13/HOXA11-AS axis regulates CCL2/CCR2, ITGAV, ITGB1, and IBSP signaling.
  • Secreted HOXA11-AS from PC3 cells induces CCL2 and IBSP expression in SaOS2 osteoblastic cells.

Conclusions:

  • Prostate cancer HOXA11-AS and HOXB13 promote metastasis.
  • They regulate metastasis through autocrine and paracrine actions on cytokine and integrin signaling pathways.
Keywords:
HOXA11-ASHOXB13long noncoding RNAmetastasisprostate cancer

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