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Updated: Nov 19, 2025

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
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Bioactivity reinforced surface patch bound collagen-pectin hydrogel.

Himansh Goel1, Nidhi Gupta2, Deenan Santhiya1

  • 1Department of Applied Chemistry, Delhi Technological University, New Delhi, India.

International Journal of Biological Macromolecules
|January 30, 2021
PubMed
Summary

This study developed a novel collagen/pectin hydrogel with bioactive glass particles, creating an integrative 3D cell environment. The composite hydrogel shows promise for tissue regeneration and drug delivery applications.

Keywords:
Amphotericin-BBioactive glassBioactivityHybrid composite hydrogelSurface-patch binding

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Drug Delivery

Background:

  • Developing advanced biomaterials is crucial for creating realistic 3D cell environments.
  • Collagen and pectin are biocompatible polymers with potential for hydrogel formation.
  • Bioactive glass particles can enhance hydrogel properties and promote tissue regeneration.

Purpose of the Study:

  • To design and characterize a novel collagen/pectin hybrid composite hydrogel (CPBG) incorporating in-situ mineralized bioactive glass (BG) particles.
  • To evaluate the CPBG's potential for simulating an integrative 3D cell environment.
  • To assess the CPBG's suitability for drug delivery and tissue regeneration applications.

Main Methods:

  • Systematic analysis of collagen/pectin sol interactions using Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
  • Characterization of bioactive glass mineralization and integration using Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX), and Transmission Electron Microscopy (TEM).
  • In vitro bioactivity, drug release kinetics, rheological analysis, and cell viability studies with U2OS and HaCaT cell lines.

Main Results:

  • Collagen and pectin molecules formed a hybrid crosslinked network with BG nanoparticles acting as pseudo crosslink junctions.
  • In vitro tests confirmed the formation of hydroxyapatite microcrystals on the CPBG surface, indicating bioactivity.
  • The hydrogel demonstrated controlled drug release, viscoelastic compatibility, and good cell viability, with preferential cell adhesion on the hydroxyapatite layer.

Conclusions:

  • The developed collagen/pectin hybrid composite hydrogel with bioactive glass offers a promising platform for integrative 3D cell environments.
  • The CPBG exhibits excellent potential for both drug delivery and hard/soft tissue regeneration applications.
  • The study highlights the synergistic interaction between collagen, pectin, and bioactive glass for enhanced biomaterial performance.