Electrophysiological field potential identification of an intact GABAergic system in mouse cortical slices

In brain slice experiments there's currently no validated electrophysiological method for differentiating viability between GABAergic and glutamatergic cell populations. Here we investigated the neurophysiology of high frequency field potential activity - and its utility for probing the functional state of the GABAergic system in brain slices. Field potentials were recorded from mouse cortical slices exposed to 50 mM potassium ("elevated-K") and the induced high frequency (>20 Hz) response characterized pharmacologically. The elevated-K responses were also related to the high frequency activity imbedded in no-magnesium seizure-like events (SLE) from the same slices. The elevated-K response, comprising a transient burst of high frequency activity, was strongly GABA_A-dependent. The size of the high frequency response was reduced by 71% (p = 0.001) by picrotoxin, but not significantly attenuated by either APV or CNQX. High frequency activity embedded in no-magnesium SLEs correlated with the elevated-K response. The success rate for generating an elevated-K response - and high frequency SLE activity - declined rapidly with increasing time since slicing. These findings support the hypothesis that in cortical slices, a functioning synaptic GABAergic system is evidenced by a strong high frequency component to no-magnesium SLE activity - and that the integrity of the GABAergic system degrades quicker than the excitatory glutamatergic system in this preparation.