A Novel Orthotopic Mouse Model of Lung Metastasis Using Fluorescent Patient-derived Osteosarcoma Cells

Area of Science:

• Oncology
• Translational Research
• Animal Models

Background:

• Metastatic osteosarcoma is a challenging disease with limited treatment options.
• Developing effective mouse models is crucial for identifying new therapeutic agents.
• Osteosarcoma patient-derived cells (OS-PDCs) were utilized for model development.

Purpose of the Study:

• To establish a patient-derived orthotopic cell (PDOC) model for metastatic osteosarcoma in mice.
• To validate the model's ability to replicate clinical features of osteosarcoma lung metastasis.
• To provide a platform for preclinical drug screening.

Main Methods:

• Osteosarcoma patient-derived cells were transfected with green fluorescent protein (GFP) lentivirus.
• GFP-expressing OS-PDCs were injected into the tibia of nude mice to create the PDOC model.
• Primary tumors and organs were resected and imaged to assess tumor establishment and metastasis.

Main Results:

• Orthotopic primary tumors were successfully established in 2 out of 3 mice.
• Green fluorescent protein (GFP) expression allowed visualization of OS-PDCs within the PDOC model.
• Lung metastases expressing GFP were detected in one mouse with a primary tumor.

Conclusions:

• The developed GFP-expressing PDOC model effectively mimics human lung-metastatic osteosarcoma.
• This preclinical model serves as a valuable tool for evaluating potential drugs targeting osteosarcoma lung metastasis.