Related Experiment Video
Updated: Nov 19, 2025

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
Published on: July 21, 2018
A circGLIS3/miR-644a/PTBP1 positive feedback loop promotes the malignant biological progressions of non-small cell
Zhixiong Wu1, Hong Jiang1, Hong Fu1
1Department of Oncology, Tongji Hospital of Tongji University Shanghai, China.
Abstract:
Non-small cell lung cancer (NSCLC) is a severe cancer which critically threatens human health in the world. Circular RNAs (circRNAs) are non-coding RNAs that involve in cancer progression. We want to explore the roles of circRNAs in NSCLC in this study. In current study, circGLIS3 was found to be highly expressed in NSCLC tissues and cell lines and high circGLIS3 level was correlated to malignant characteristics and poor prognosis of NSCLC. Functional experiments suggested that circGLIS3 promoted proliferation, migration and invasion and arrested apoptosis of NSCLC cells in vitro. CircGLIS3 also participated in the in vivo process by accelerate NSCLC tumor growth and metastasis. Mechanistically, circGLIS3 could sponging multiple anti-cancer miRNAs including miR-526b, miR-198, miR-498 and miR-664a. Here, we for the first time confirmed that miR-644a was downregulated and functioned as a tumor suppression gene in NSCLC. In addition, we found PTBP1 as a novel target of miR-644a and circGLIS3 could raise the expression of PTBP1 via miR-644a. And PTBP1 could bind to the flanking introns of circGLIS3 and thereby promoting looping of circGLIS3. In conclusion, CircGLIS3 functions as an oncogene via sponging multiple tumor-suppressive miRNAs in NSCLC. A circGLIS3/miR-644a/PTBP1 positive feedback loop exists in the tumorigenesis and development of NSCLC.
More Related Videos
13:18Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
Published on: March 3, 2023
06:52Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence
Published on: November 5, 2021
Related Concept Videos
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Tumor Progression
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
PI3K/mTOR/AKT Signaling Pathway
Abnormal Proliferation
Positive Regulator Molecules
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...