Detection of singlet oxygen luminescence for experimental corneal rose bengal photodynamic antimicrobial therapy

Rose bengal photodynamic antimicrobial therapy (RB-PDAT) treats corneal infection by activating rose bengal (RB) with green light to produce singlet oxygen (¹O₂). Singlet oxygen dosimetry can help optimize treatment parameters. We present a ¹O₂ dosimeter for detection of ¹O₂ generated during experimental RB-PDAT. The system uses a 520 nm laser and an InGaAs photoreceiver with bandpass filters to detect ¹O₂ luminescence during irradiation. The system was validated in RB solutions and ex vivo in human donor eyes. The results demonstrate the feasibility of ¹O₂ dosimetry in an experimental model of RB-PDAT in the cornea.