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Multi-layer Shack-Hartmann wavefront sensing in the point source regime.

Vyas Akondi1, Alfredo Dubra1

  • 1Byers Eye Institute, Stanford University, Palo Alto, California 94303, USA.

Biomedical Optics Express
|February 1, 2021
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Summary
This summary is machine-generated.

Artifactual aberrations in Shack-Hartmann wavefront sensors (SHWS) arise from multi-layered samples. We propose a minimum integration area to reduce these aberrations, improving retinal imaging and microscopy.

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Area of Science:

  • Biomedical Optics
  • Ophthalmic Imaging
  • Optical Metrology

Background:

  • Shack-Hartmann wavefront sensors (SHWS) typically assume single wavefront detection.
  • Multi-layered biological tissues can generate secondary wavefronts, causing artifactual aberrations.
  • These aberrations impact the accuracy of SHWS-based measurements in biological samples.

Purpose of the Study:

  • To evaluate artifactual aberrations in a simulated ophthalmic SHWS.
  • To model wavefront beacons from a two-layer retina in human and mouse eyes.
  • To develop a method for mitigating SHWS-induced aberrations.

Main Methods:

  • Simulated Shack-Hartmann wavefront sensing of a two-layer retinal model.
  • Modeling of beacon generation from displaced retinal regions.
  • Derivation of formulae for optimal SHWS centroid integration area.

Main Results:

  • Secondary wavefronts from a two-layer retina introduce significant artifactual aberrations.
  • The proposed formulae provide a minimum integration area to mitigate these aberrations.
  • Mitigation by an order of magnitude is achievable with the proposed method.

Conclusions:

  • Artifactual aberrations are a significant concern for SHWS in multi-layered biological samples.
  • Calculating a minimum SHWS centroid integration area can effectively reduce these aberrations.
  • This approach can enhance the performance of SHWS-based metrology and adaptive optics systems for retinal imaging and microscopy.