Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinetics of endothelial cell-surface attachment forces.

K J Pratt1, B E Jarrell, S K Williams

  • 1Department of Surgery, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.

Journal of Vascular Surgery
|April 1, 1988
PubMed
Summary

Optimizing incubation time and protein substrates enhances endothelial cell (EC) attachment to blood vessel surfaces. This allows for rapid formation of durable, biocompatible EC monolayers under shear stress conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rationale and design of a pilot study to evaluate the acceptability and effectiveness of a revised protein sparing modified fast (rPSMF) for severe obesity in a pediatric tertiary care weight management clinic.

Contemporary clinical trials communications·2019
Same author

Uncontrolled blood pressure and risk of sleep apnea among blacks: findings from the Metabolic Syndrome Outcome (MetSO) study.

Journal of human hypertension·2015
Same author

Survey of management of common iliac artery aneurysms by members of the Vascular Society of Great Britain and Ireland.

Annals of the Royal College of Surgeons of England·2014
Same author

Effects of packaging systems and fat concentrations on microbiology, sensory and physical properties of ground beef stored at 4±1°C for 25 days.

Meat science·2014
Same author

Quantification of hepatic FOXP3+ T-lymphocytes in HIV/hepatitis C coinfection.

Journal of viral hepatitis·2014
Same author

Prenatal and gestational cocaine exposure: Effects on the oxytocin system and social behavior with implications for addiction.

Pharmacology, biochemistry, and behavior·2013

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Hemodynamics

Background:

  • Endothelial cells (ECs) are crucial for blood vessel function, requiring persistent attachment to native and prosthetic surfaces.
  • Optimizing EC attachment forces is key for establishing durable, biocompatible EC monolayers rapidly.

Purpose of the Study:

  • To investigate the effects of protein substrate, incubation time, and shear stress on human adult EC attachment kinetics.
  • To determine optimal conditions for rapid EC monolayer formation on polymer surfaces.

Main Methods:

  • Human adult ECs were incubated on polystyrene (PS) or polyethylene terephthalate (PET) polymers coated with various extracellular matrix proteins (collagen I/III, fibronectin, collagen IV/V, laminin, gelatin) or saline control for up to 30 minutes.
  • Attachment was evaluated after exposure to shear stress (0-90 dynes/cm²) in a rotating disc device for 30 minutes.

Related Experiment Videos

  • Shear-resistant adherence was quantified by measuring the percentage of attached cells post-shear stress relative to pre-shear exposure.

    • Main Results:

    • Maximal EC adherence was observed on all tested substrates by 30 minutes of incubation.
    • ECs attached to fibronectin or collagen I/III matrices showed shear-resistant adherence after only 5 minutes of static incubation.
    • After 30 minutes, over 90% of ECs on fibronectin and collagen I/III matrices remained attached under 90 dynes/cm² shear stress.

    Conclusions:

    • EC attachment forces are influenced by incubation time and substrate composition.
    • A defined shear stress detachment assay can quantify these attachment forces.
    • Understanding and manipulating these physiochemical parameters can facilitate the creation of optimal EC monolayers on blood-contacting surfaces.