Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Publisher Correction: In situ nanocrystal confinement for efficient blue perovskite LEDs.

Nature·2026

Same author

Spatial double-shelled structure achieving stable anion exchange membrane water electrolysis via enhanced local alkalinity.

Science advances·2026

Same author

In situ nanocrystal confinement for efficient blue perovskite LEDs.

Nature·2026

Same author

Atomic-scale revelation of in situ reverse regulation from particles to clusters in the Ni/La-CeO<sub>x</sub> catalyst.

Nature communications·2026

Same author

Interface-enhanced Ni<sub>3</sub>S<sub>2</sub>/LaNiO<sub>3</sub> heterostructure for efficient water oxidation.

Chemical communications (Cambridge, England)·2026

Same author

Harnessing rare earth coordination chemistry for advanced photofunctional materials: from fundamental principles to emerging technologies.

Chemical Society reviews·2026

Same journal

An integrated annotation strategy for the phytochemical characterization of Xie-Bai-San decoction based on UPLC-Q Exactive Orbitrap HRMS, multi-database screening, and feature-based molecular networking.

Frontiers in chemistry·2026

Same journal

Core-shell structured nanomaterials in dual-modal magnetic resonance imaging guided antitumor effect via combined treatment.

Frontiers in chemistry·2026

Same journal

Photo-responsive nanozymes: from photocatalytic mechanisms to precision therapy.

Frontiers in chemistry·2026

Same journal

From theoretical energy to practical utilization: interfacial stability, transport kinetics, and cell-level design in high-energy lithium-metal batteries.

Frontiers in chemistry·2026

Same journal

Zinc-vacancy defects in ZnO nanorods induced visible-light activity of photoelectrochemical glucose sensing: experimental and DFT+U analysis.

Frontiers in chemistry·2026

Same journal

Integrating multi-isotope calibration and infrared-assisted digestion for robust and sustainable multielemental determination in agroalimentary matrices by ICP-MS.

Frontiers in chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 19, 2025

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy.

Hao Dong¹, Ling-Dong Sun¹, Chun-Hua Yan^1,2

¹Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

Frontiers in Chemistry

|February 1, 2021

Summary

This summary is machine-generated.

Lanthanide-doped upconversion nanoparticles offer unique optical properties for super-resolution microscopy. These advanced probes show promise for high-resolution imaging of subcellular structures, advancing cell biology applications.

Keywords:

STED lanthanide multiphoton imaging super-resolution microscopy upconversion nanoparticle

More Related Videos

Triplet Fusion Upconversion Nanocapsule Synthesis

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

Related Experiment Videos

Last Updated: Nov 19, 2025

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Triplet Fusion Upconversion Nanocapsule Synthesis

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

Area of Science:

Nanotechnology
Optical Microscopy
Biophysics

Background:

Super-resolution microscopy requires advanced luminescent probes for nanoscale imaging.
Lanthanide-doped upconversion nanoparticles (UCNPs) possess unique optical properties like photostability and tunable emissions.
UCNPs are emerging as promising probes for super-resolution imaging studies.

Purpose of the Study:

To summarize recent advances in using UCNPs for super-resolution microscopy.
To project future directions for UCNP-based super-resolution imaging.
To guide the design of efficient UCNP nanoprobes for cell biology.

Main Methods:

Review of existing literature on UCNPs in super-resolution microscopy.
Analysis of UCNP optical properties relevant to imaging.
Discussion of current resolution limits and future potential.

Main Results:

UCNPs have achieved resolutions of 28 nm for single nanoparticles and 82 nm for cytoskeleton structures.
UCNP-based super-resolution microscopy is still in its early stages compared to conventional probes.
UCNPs offer advantages such as photostability and large anti-Stokes shifts.

Conclusions:

UCNPs represent a developing area with significant opportunities for super-resolution imaging.
Further development of UCNPs is crucial for advancing cell biology applications.
This perspective highlights the potential of UCNPs to overcome current imaging limitations.