Related Experiment Video
Updated: Nov 19, 2025
11:44
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
7.8K
MICAL-L2 Is Essential for c-Myc Deubiquitination and Stability in Non-small Cell Lung Cancer Cells
Pengxiang Min1,2, Lin Zhang3, Yueyuan Wang1
1Department of Physiology, Nanjing Medical University, Nanjing, China.
Frontiers in Cell and Developmental Biology
|February 1, 2021
Summary
MICAL-L2 promotes non-small cell lung cancer (NSCLC) proliferation by stabilizing the c-Myc protein. This study reveals MICAL-L2
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- MICAL-L2 (Molecules Interacting with CasL-2) is upregulated in various cancers.
- Its specific role in non-small cell lung cancer (NSCLC) pathogenesis is not well understood.
Purpose of the Study:
- To elucidate the mechanisms by which MICAL-L2 influences NSCLC cell proliferation.
- To investigate the interaction between MICAL-L2 and c-Myc in NSCLC.
Main Methods:
- Immunohistochemistry to assess MICAL-L2 expression in NSCLC tissues.
- Cell transfection (siRNA/plasmids) to modulate MICAL-L2 levels.
- EdU staining, CCK-8 assays, Western blotting, immunofluorescence, co-immunoprecipitation, and protein stability assays to analyze proliferation, cell cycle, and protein interactions.
Main Results:
- MICAL-L2 is highly expressed in NSCLC and promotes cell proliferation.
- MICAL-L2 interacts with c-Myc, maintaining its nuclear levels and prolonging its half-life.
- MICAL-L2 deubiquitinates c-Myc, inhibiting its degradation and potentially blocking phosphorylation at Thr58.
Conclusions:
- MICAL-L2 acts as a crucial regulator of c-Myc deubiquitination and stability within the nucleus.
- This regulatory function of MICAL-L2 contributes to the promotion of NSCLC cell proliferation.
More Related Videos
Related Concept Videos
02:23Abnormal Proliferation
4.9K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
01:06Induced Pluripotent Stem Cells
4.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.9K
01:22PI3K/mTOR/AKT Signaling Pathway
4.7K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.7K
02:23Master Transcription Regulators
7.4K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.4K
00:50Anaphase Promoting Complex
3.1K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.1K
02:15M-Cdk Drives Transition Into Mitosis
6.0K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.0K