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Updated: Nov 19, 2025

Bovine Ovarian Cortex Tissue Culture

Bovine Ovarian Cortex Tissue Culture

Published on: January 14, 2021

Bovine Ovarian Cortex Tissue Culture.

Courtney M Sutton¹, Shelby A Springman¹, Mohamed A Abedal-Majed²

¹Department of Animal Science, University of Nebraska-Lincoln.

Journal of Visualized Experiments : Jove

|February 1, 2021

Summary

The Stair-Step diet may enhance bovine ovarian follicle development by influencing steroid hormones within the ovarian microenvironment during in vitro culture. This nutritional approach shows promise for fertility research.

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Area of Science:

Reproductive Biology
Animal Science
Endocrinology

Background:

Follicle development is crucial for fertility and influenced by the ovarian microenvironment.
The impact of specific nutritional strategies on the ovarian microenvironment and follicle progression remains understudied.
In vitro ovarian cortex culture offers a controlled environment to study these effects.

Purpose of the Study:

To investigate the effect of the Stair-Step diet on bovine ovarian microenvironment, including follicle development, steroid, and cytokine production.
To evaluate the utility of in vitro ovarian cortex culture for assessing nutritional impacts on ovarian function.

Main Methods:

Bovine ovarian cortical pieces from heifers on Control or Stair-Step diets were cultured in vitro for 7 days.

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Last Updated: Nov 19, 2025

Bovine Ovarian Cortex Tissue Culture

Bovine Ovarian Cortex Tissue Culture

Published on: January 14, 2021

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

Published on: July 8, 2020

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

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Follicle development was assessed histologically before and after culture.

Steroids, steroid metabolites, and cytokines in culture media were measured.

Main Results:

A tendency for increased steroid hormones was observed in the ovarian microenvironment of the Stair-Step group compared to the Control group.
These hormonal changes correlated with potential follicle progression.

Conclusions:

In vitro ovarian cortex culture is a valuable tool for understanding the ovarian microenvironment and the effects of nutrition on follicle development.
The Stair-Step nutritional strategy shows potential for positively influencing ovarian steroidogenesis and follicle progression.
This model system could aid in developing fertility-enhancing therapeutics.