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Related Experiment Video

Updated: Nov 19, 2025

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

Fully Automated Ancestral Sequence Reconstruction using FireProt^ASR.

Rayyan Tariq Khan^1,2, Milos Musil^1,2,3, Jan Stourac^1,2

¹Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic.

Current Protocols

|February 1, 2021

Summary

This summary is machine-generated.

Ancestral sequence reconstruction (ASR) automates protein evolution analysis. This technique enhances protein thermostability and substrate specificity for biotechnology and medicine.

Keywords:

ancestral sequence reconstruction automation protein engineering protein evolution thermostability

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Published on: March 12, 2012

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Last Updated: Nov 19, 2025

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

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Published on: July 11, 2025

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

The ITS2 Database

The ITS2 Database

Published on: March 12, 2012

Area of Science:

Protein engineering
Biotechnology
Evolutionary biology

Background:

Protein evolution and engineering are crucial for basic science and industrial applications.
Ancestral sequence reconstruction (ASR) is a key technique for understanding evolutionary relationships and engineering proteins with improved properties like thermostability and substrate specificity.

Purpose of the Study:

To describe the setup and execution of an automated FireProtASR workflow.
To provide a user-friendly web service for inferring ancestral proteins from various input types.

Main Methods:

Automated workflow using the FireProtASR web server.
Input flexibility: single sequence, homologous sequences, multiple sequence alignment (MSA), or phylogenetic tree.
Automatic dataset building, MSA, phylogenetic tree construction, and ancestral node reconstruction.

Main Results:

Successful automation of all necessary steps for ASR.
Enables novices to improve protein properties, including catalytic promiscuity.
Provides a freely accessible web server for ASR: https://loschmidt.chemi.muni.cz/fireprotasr/.

Conclusions:

The FireProtASR workflow simplifies and automates ancestral protein reconstruction.
This tool enhances protein engineering capabilities for research and industry.
Facilitates the development of proteins with desirable characteristics for diverse applications.