Dual-laser measurement of human stapes footplate motion under blast exposure

Hearing damage is one of the most frequently observed injuries in Service members and Veterans even though hearing protection devices (HPDs, e.g. earplugs) have been implemented to prevent blast-induced hearing loss. However, the formation and prevention mechanism of the blast-induced hearing damage remains unclear due to the difficulty for conducting biomechanical measurements in ears during blast exposure. Recently, an approach reported by Jiang et al. (2019) used two laser Doppler vibrometers (LDVs) to measure the motion of the tympanic membrane (TM) in human temporal bones during blast exposure. Using the dual laser setup, we further developed the technology to detect the movement of the stapes footplate (SFP) in ears with and without HPDs while under blast exposure. Eight fresh human cadaveric temporal bones (TBs) were involved in this study. The TB was mounted in a "head block" after performing a facial recess surgery to access the SFP, and a pressure sensor was inserted near the TM in the ear canal to measure the pressure reaching the TM (P1). The TB was exposed to a blast overpressure measuring around 7 psi or 48 kPa at the entrance of the ear canal (P0). Two LDVs were used to measure the vibrations of the SFP and TB (as a reference). The exact motion of the SFP was determined by subtracting the TB motion from the SFP data. Results included a measured peak-to-peak SFP displacement of 68.7 ± 31.6 μm (mean ± SD) from all eight TBs without HPDs. In five of the TBs, the insertion of a foam earplug reduced the SFP displacement from 48.3 ± 6.3 μm to 21.8 ± 10.4 μm. The time-frequency analysis of the SFP velocity signals indicated that most of the energy spectrum was concentrated at frequencies below 4 kHz within the first 2 ms after blast and the energy was reduced after the insertion of HPDs. This study describes a new methodology to quantitatively characterize the response of the middle ear and the energy entering the cochlea during blast exposure. The experimental data are critical for determining the injury of the peripheral auditory system and elucidating the damage formation and prevention mechanism in an ear exposed to blast.