Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Liver Regeneration01:24

Liver Regeneration

3.9K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.9K
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow01:26

Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow

104
Chronic liver disease significantly impacts drug metabolism due to alterations in hepatic blood flow and enzyme accessibility. This disruption affects the body's pharmacokinetics—the movement and processing of drugs within the system. Key enzymes crucial for metabolizing medications become less accessible, changing how drugs are processed and utilized. Furthermore, liver disease influences the synthesis of plasma proteins, such as albumin and globulins, which play critical roles in drug...
104

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of Microvascular Failure Using Bioimpedance and Sensor-derived Clinical Indicators: A Preliminary Study.

Plastic and reconstructive surgery. Global open·2026
Same author

"Hope" on the Horizon-But Are We Asking the Right Questions?

JAMA surgery·2026
Same author

Acellular Normothermic Machine Perfusion Does not Exacerbate Acute Rejection in Rodent Vascularized Composite Allografts.

Transplantation direct·2026
Same author

Development and psychometric evaluation of the Liver Disease Stigma Scale (LDSS).

JHEP reports : innovation in hepatology·2026
Same author

Impact of donor and recipient sex on graft function among deceased donor kidney recipients: A paired-kidney analysis.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Liver bioengineering for regeneration: advances in liver disease modeling approaches and decellularization strategies.

Regenerative medicine·2026

Related Experiment Video

Updated: Nov 19, 2025

Decellularization and Recellularization of Whole Livers
09:24

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

22.3K

Liver donor age affects hepatocyte function through age-dependent changes in decellularized liver matrix.

Aylin Acun1, Ruben Oganesyan1, Korkut Uygun1

  • 1Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children, Boston, MA, USA; Department of Surgery, Massachusetts General Hospital, Boston, MA, USA.

Biomaterials
|February 1, 2021
PubMed
Summary

Donor liver quality declines with age, impacting its suitability for transplantation and tissue engineering. Older liver extracellular matrix (ECM) shows detrimental changes affecting cell function, highlighting donor age as a critical factor for bioengineered liver substitutes.

Keywords:
AgingDecellularizationExtracellular matrixLiver

More Related Videos

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
05:20

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

3.3K
3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
06:46

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

2.1K

Related Experiment Videos

Last Updated: Nov 19, 2025

Decellularization and Recellularization of Whole Livers
09:24

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

22.3K
Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy
05:20

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

3.3K
3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
06:46

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

2.1K

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Hepatology
  • Biomaterials Science

Background:

  • Orthotopic liver transplantation is the sole treatment for end-stage liver disease, facing significant donor organ scarcity.
  • Discarded donor livers present an opportunity for decellularization and recellularization, but scaffold quality is crucial.
  • Donor age is a potential factor influencing the extracellular matrix (ECM) quality of decellularized liver scaffolds.

Purpose of the Study:

  • To investigate age-dependent alterations in the liver extracellular matrix (ECM).
  • To assess the impact of these age-related ECM changes on primary hepatocyte function.
  • To determine the implications of ECM modifications for bioengineering liver substitutes.

Main Methods:

  • Comparative analysis of liver ECM composition (collagen, glycosaminoglycans, growth factors) in young and aged rat and human liver samples.
  • Assessment of primary hepatocyte function cultured on decellularized liver scaffolds of varying ages.
  • Evaluation of ECM stiffening and integrin-dependent signaling pathways.

Main Results:

  • Aging liver ECM exhibits increased collagen and glycosaminoglycan content.
  • A decrease in growth factor content was observed in older liver ECM.
  • Primary hepatocyte function deteriorated on aged scaffolds, linked to ECM stiffening and altered integrin signaling.

Conclusions:

  • Liver ECM composition and properties change significantly with donor age.
  • These age-related ECM changes negatively impact cellular function, crucial for tissue engineering.
  • Donor age must be a key consideration when developing bioengineered liver substitutes from decellularized scaffolds.