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MicroRNA-125a-5p modulates macrophage polarization by targeting E26 transformation-specific variant 6 gene during

Wendan He1, Nan Zhang2, Zhengshen Lin3

  • 1Department of Stomatology, Shenzhen Hospital, Southern Medical University, Shenzhen, 518033, China.

Archives of Oral Biology
|February 1, 2021
PubMed
Abstract

Insights

MicroRNA-125a-5p promotes bone healing during orthodontic tooth movement by enhancing M2 macrophage polarization. This involves targeting the ETV6 gene, crucial for regulating macrophage phenotypes and facilitating tissue repair.

Area of Science:

  • Molecular Biology
  • Immunology
  • Orthodontics

Background:

  • Orthodontic tooth movement (OTM) involves complex cellular and molecular processes in the periodontal ligament.
  • Macrophage polarization plays a critical role in modulating inflammatory responses and tissue remodeling during OTM.
  • The specific role of microRNA-125a-5p (miR-125a-5p) in macrophage behavior during OTM remains largely unexplored.

Purpose of the Study:

  • To investigate the role of miR-125a-5p in macrophages during the process of orthodontic tooth movement.
  • To elucidate the molecular mechanisms by which miR-125a-5p influences macrophage polarization.
  • To determine the therapeutic potential of targeting miR-125a-5p for enhancing bone healing in OTM.

Main Methods:

  • Isolation and culture of periodontal ligament cells and stem cells from human tissues.
  • Quantification of miR-125a-5p levels using real-time quantitative PCR.
  • Assessment of macrophage polarization (M1/M2) using alizarin red staining, flow cytometry, Western blot, and gene expression analysis.
  • Verification of the interaction between miR-125a-5p and its target gene ETV6 using luciferase reporter and RNA immunoprecipitation assays.

Main Results:

  • miR-125a-5p expression was significantly upregulated in periodontal tissues under orthodontic force and in M2-polarized macrophages.
  • Downregulation of miR-125a-5p promoted M1 macrophage markers while suppressing M2 markers.
  • ETV6 was identified as a direct target of miR-125a-5p; ETV6 overexpression mimicked the effects of miR-125a-5p inhibition on macrophage polarization.

Conclusions:

  • miR-125a-5p plays a crucial role in promoting M2 macrophage polarization during orthodontic tooth movement.
  • The mechanism involves miR-125a-5p targeting ETV6, thereby influencing macrophage phenotype.
  • These findings suggest that miR-125a-5p is a potential therapeutic target for enhancing bone healing in orthodontic treatments.
Keywords:
ETV6Macrophage polarizationmiR-125a-5p

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