Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

6.9K
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harmonization of measurement units: mission impossible or ethical imperative?

Clinical chemistry and laboratory medicine·2026
Same author

Prospective evaluation of the effect of stopping estrogen-containing contraceptives on serum CBG and cortisol levels.

The Journal of clinical endocrinology and metabolism·2026
Same author

National comparability and inter-laboratory performance of cortisol immunoassays and LC-MS/MS methods.

Clinical chemistry and laboratory medicine·2026
Same author

The value-based score in laboratory medicine: results of a European pilot study.

Clinical chemistry and laboratory medicine·2026
Same author

Calcium-conditioned PTH prediction intervals are promising but not yet transferable to routine practice.

Clinical chemistry and laboratory medicine·2026
Same author

Accuracy-Based Proficiency Testing Surveys for Testosterone Provide Important Information About Measurement Accuracy and Its Impact on Patient Care.

Clinical chemistry·2026

Related Experiment Video

Updated: Nov 19, 2025

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
06:43

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents

Published on: August 31, 2013

18.9K

Analytical Performance Specifications for 25-Hydroxyvitamin D Examinations.

Etienne Cavalier1, Callum G Fraser2, Harjit P Bhattoa3

  • 1CHU de Liège and Centre de Recherche Intégré sur les Médicaments (CIRM), Department of Clinical Chemistry, University of Liège, Domaine du Sart-Tilman, B-4000 Liège, Belgium.

Nutrients
|February 2, 2021
PubMed
Summary
This summary is machine-generated.

Measuring 25-hydroxy vitamin D (25(OH)D) is complex. This study found participants were not in a steady state, suggesting measurement uncertainty-based analytical performance specifications are more appropriate than biological variation-based ones for vitamin D status.

Keywords:
25(OH)-vitamin Danalytical performance specifications biological variationmeasurement uncertaintyvitamin D

More Related Videos

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling
05:03

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling

Published on: December 27, 2024

1.4K
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

41.1K

Related Experiment Videos

Last Updated: Nov 19, 2025

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
06:43

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents

Published on: August 31, 2013

18.9K
Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling
05:03

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling

Published on: December 27, 2024

1.4K
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

41.1K

Area of Science:

  • Clinical Chemistry
  • Biomarker Analysis
  • Vitamin D Metabolism

Background:

  • 25-hydroxy vitamin D (25(OH)D) is the primary indicator of vitamin D status.
  • Accurate measurement of 25(OH)D is crucial but technically challenging.
  • Establishing reliable analytical performance specifications (APS) is essential for clinical interpretation.

Purpose of the Study:

  • To evaluate the biological variation (BV) of 25(OH)D to inform the setting of APS for measurement uncertainty (MU).
  • To assess the steady-state assumption in participants for BV analysis.
  • To determine appropriate APS for 25(OH)D assays.

Main Methods:

  • Collected K3-EDTA plasma weekly for up to 10 weeks from 91 healthy participants across six European labs.
  • Analyzed 25(OH)D concentrations in duplicate using a Lumipulse G1200 analyzer.
  • Assessed biological variation (within- and between-subject) and participant steady-state.

Main Results:

  • Participants were not in a steady state during the 10-week collection period.
  • Within-subject BV estimates ranged from 5.8% to 7.1%, and between-subject BV ranged from 25.0% to 39.2%.
  • Required MU for detecting 25(OH)D supplementation effects at p < 0.05 was <13.6%, and at p < 0.01 was <9.6%.

Conclusions:

  • The assumption of a steady state for BV-based APS development is not valid for 25(OH)D.
  • Patient samples may not be in a consistent physiological state for BV analysis.
  • Analytical performance specifications based on measurement uncertainty are more suitable for 25(OH)D assays.