Pseudomonas bijieensis sp. nov., isolated from cornfield soil

Strain L22-9^T, a Gram-stain-negative and rod-shaped bacterium, motile by one polar flagellum, was isolated from cornfield soil in Bijie, Guizhou Province, PR China. Based on 16S rRNA gene sequences, it was identified as a Pseudomonas species. Multilocus sequence analysis of concatenated 16S rRNA, gyrB, rpoB and rpoD gene sequences showed that strain L22-9^T formed a clearly separated branch, located in a cluster together with Pseudomonas brassicacearum LMG 21623^T, Pseudomonas kilonensis DSM 13647^T and Pseudomonas thivervalensis DSM 13194^T. Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) confirmed that strain L22-9^T should be classified as a novel species. It was most closely related to P. kilonensis DSM 13647^T with ANI and dDDH values of 91.87 and 46.3 %, respectively. Phenotypic features that can distinguish strain L22-9^T from P. kilonensis DSM 13647^T are the assimilation ability of N-acetyl-d-glucosamine, poor activity of arginine dihydrolase and failure to ferment ribose and d-fucose. The predominant cellular fatty acids of strain L22-9^T are C_16 : 0, summed feature 3 (C_16 : 1 ω6c and/or C_16 : 1 ω7c) and summed feature 8 (C_18 : 1 ω7c and/or C_18 : 1 ω6c). The respiratory quinones consist of Q-9 and Q-8. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phosphoglycolipids, two unidentified aminophospholipids and an unidentified glycolipid. Based on the evidence, we conclude that strain L22-9^T represents a novel species, for which the name Pseudomonas bijieensis sp. nov. is proposed. The type strain is L22-9^T (=CGMCC 1.18528^T=LMG 31948^T), with a DNA G+C content of 60.85 mol%.