Neonicotinoid residues in honey from urban and rural environments

Pesticide residues in honey can negatively affect bee health. Although recent studies have detected neonicotinoid residues in honeys from around the world, little is known about how residues relate to land use and vegetation composition. To investigate potential relationships, we sampled multi-floral honey from 30 Apis mellifera hives from urban, agricultural and semi-natural habitats (SNH), identified and quantified three neonicotinoids present (clothianidin, imidacloprid and thiacloprid) using UHPLC-MS, and classified surrounding land use up to 5 km around hive sites. Neonicotinoids were most frequently detected in honeys from hives in agricultural habitats, and 70% of all samples contained at least one of the three neonicotinoid compounds. Imidacloprid was the most frequently detected neonicotinoid (found in 43% of honey samples) followed by clothianidin (40%) and thiacloprid (37%). Almost half (48%) of samples contained at least two neonicotinoids, and two of the 30 samples contained all three. Clothianidin and thiacloprid were more frequently detected in honeys from urban habitats, highlighting that exposure to pesticides does not just occur in agricultural settings. This suggests that pesticide use in urban domestic, sport and amenity contexts, given potential exposure of bees and other pollinators, needs urgent consideration.