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Updated: Nov 19, 2025

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SIRT5 regulates autophagy and apoptosis in gastric cancer cells

Wen Gu1, Qinyi Qian2, Yinkai Xu1

  • 1Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou, China.

The Journal of International Medical Research
|February 3, 2021
PubMed
Abstract

Insights

Sirtuin 5 (SIRT5) is downregulated in gastric cancer (GC) and promotes autophagy via the AMPK-mTOR pathway. SIRT5 also regulates apoptosis, maintaining a balance crucial for GC cells.

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • Sirtuins (SIRTs) are implicated in cancer cell regulation.
  • The specific role of SIRT5 in gastric cancer (GC) remains uncharacterized.

Purpose of the Study:

  • To investigate the function of SIRT5 in gastric cancer (GC) cells.
  • To elucidate SIRT5's role in regulating autophagy and apoptosis in GC.

Main Methods:

  • SIRT5 protein levels were assessed in GC patient samples.
  • Autophagy promotion by SIRT5 was studied in GC cells.
  • The AMP-activated protein kinase-mammalian target of rapamycin (AMPK-mTOR) pathway was investigated.
  • GC cell apoptosis models were used to analyze SIRT5's role in apoptosis.

Main Results:

  • SIRT5 expression was found to be downregulated in GC tissues.
  • SIRT5 was demonstrated to promote autophagy in GC cells through the AMPK-mTOR pathway.
  • SIRT5 degradation was observed during GC cell apoptosis, involving calpains and caspase-related proteins.

Conclusions:

  • SIRT5 acts as a key regulator of both autophagy and apoptosis in GC.
  • SIRT5 plays a vital role in maintaining the balance between autophagy and apoptosis in gastric cancer cell lines.
Keywords:
AMP-activated protein kinaseSirtuin 5apoptosisautophagycalpaincaspasegastric cancermammalian target of rapamycin

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