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LINC01278 is Highly Expressed in Osteosarcoma and Participates in the Development of Tumors by Mediating the

Guo-Feng Zhang1, Bai-Sui Zhou1, Xiao-Chun An1

  • 1Department of Orthopedics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 261400, People's Republic of China.

Oncotargets and Therapy
|February 3, 2021
PubMed
Abstract

Insights

High expression of LINC01278 in osteosarcoma (OS) correlates with poor prognosis and aids diagnosis. This long non-coding RNA suppresses OS cell proliferation and metastasis via the miR-134-5p/KRAS axis, offering a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), play critical regulatory roles in osteosarcoma (OS).
  • LINC01278 is a newly identified lncRNA with elevated expression in OS, but its underlying mechanism and clinical significance require elucidation.

Purpose of the Study:

  • To investigate the mechanistic role of LINC01278 in osteosarcoma (OS).
  • To identify LINC01278 as a potential diagnostic marker and therapeutic target for OS.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for LINC01278 expression analysis and diagnostic value assessment.
  • Cellular assays (CCK-8, Transwell, flow cytometry) to evaluate OS cell proliferation, migration, invasion, and apoptosis.
  • RNA immunoprecipitation (RIP), RNA pull-down, and dual luciferase reporter assays to confirm molecular interactions.
  • In vivo experiments in nude mice to assess the effect of LINC01278 on tumor growth.

Main Results:

  • LINC01278 expression was significantly upregulated in OS tissues and serum, correlating with poor prognosis and demonstrating diagnostic value.
  • Functional studies revealed that LINC01278 inhibits OS cell proliferation and metastasis by modulating the miR-134-5p/KRAS axis.
  • In vivo studies confirmed that LINC01278 promotes OS tumor growth.

Conclusions:

  • Elevated LINC01278 expression in OS is associated with poor patient outcomes and serves as a diagnostic indicator.
  • LINC01278 exerts its oncogenic effects by suppressing proliferation and apoptosis through the miR-134-5p/KRAS pathway.
  • LINC01278 represents a promising therapeutic target for osteosarcoma treatment.
Keywords:
KRASLINC01278apoptosismiR-134-5posteosarcomaproliferation

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