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Chromatin Immunoprecipitation- ChIP

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Related Experiment Video

Updated: Nov 19, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

Protocol for assaying chromatin accessibility using ATAC-seq in plants.

Fu-Xiang Wang^1,2, Guan-Dong Shang^1,2, Lian-Yu Wu^1,3

¹National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS center for Excellence in Molecular Plant Science, Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences (CAS), Shanghai 200032, China.

|February 3, 2021

Summary

We developed a new protocol for Transposase-Accessible Chromatin Sequencing (ATAC-seq) in Arabidopsis thaliana. This method reliably detects changes in genome-wide chromatin accessibility during plant development.

Keywords:

Bioinformatics Gene Expression Model Organisms Sequencing

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Last Updated: Nov 19, 2025

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Published on: November 13, 2017

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Area of Science:

Genomics
Molecular Biology
Plant Science

Background:

Open genomic regions are crucial for gene regulation, serving as binding sites for transcription factors and chromatin modifiers.
Transposase-Accessible Chromatin Sequencing (ATAC-seq) is a powerful technique for mapping these accessible chromatin regions within intact nuclei.

Purpose of the Study:

To present a robust protocol for generating ATAC-seq libraries from fresh Arabidopsis thaliana tissues.
To establish a user-friendly bioinformatic analysis pipeline for ATAC-seq data.

Main Methods:

The protocol involves preparing ATAC-seq libraries directly from fresh plant tissues.
An accessible bioinformatic pipeline is provided for data analysis.

Main Results:

The described method enables reliable detection of chromatin accessibility changes.
This approach is applicable across different plant species and tissues.

Conclusions:

This protocol facilitates the study of dynamic changes in chromatin accessibility during plant growth and development.
The method offers a reliable tool for plant genomics research.