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Analysing endometrial microbiome: methodological considerations and recommendations for good practice.

Nerea M Molina1,2, Alberto Sola-Leyva1,2, Thor Haahr3

  • 1Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Granada, Granada 18071, Spain.

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The female reproductive tract harbors its own microbial communities, but their impact on fertility is unclear. This study outlines best practices for analyzing the endometrial microbiome to ensure accurate research findings.

Keywords:
endometrium / metagenome / microbiome / microbiota / uterus

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Area of Science:

  • Reproductive biology
  • Microbiome research
  • Gynecology

Background:

  • Emerging evidence suggests the upper female genital tract is not sterile.
  • The role of endometrial microorganisms in reproductive function requires further investigation.
  • Initial studies link the endometrial microbiome to poor reproductive outcomes and gynecological pathologies.

Purpose of the Study:

  • To highlight methodological considerations for endometrial microbiome studies.
  • To propose good practice recommendations for future research.
  • To address challenges in studying the low-biomass endometrial niche.

Main Methods:

  • Focus on methodological considerations for endometrial microbiome analysis.
  • Emphasis on well-designed and controlled experiments.
  • Strategies to avoid and adjust for contamination, particularly from the lower genital tract.

Main Results:

  • The endometrial microbiome is a complex area of study.
  • Challenges exist in analyzing low-biomass samples.
  • Contamination is a significant concern requiring careful management.

Conclusions:

  • Standardized methods are crucial for reliable endometrial microbiome research.
  • Good practices are essential to overcome contamination challenges.
  • Further research is needed to fully elucidate the impact of the endometrial microbiome on reproductive health.