Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

4.4K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
4.4K
Centrosome Duplication02:25

Centrosome Duplication

4.4K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.4K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.7K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.7K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.2K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.2K
The Structure of Intermediate Filaments01:19

The Structure of Intermediate Filaments

5.0K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
5.0K
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

2.4K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human ZBP1 is a potent inducer of cell death through mechanisms divergent from mouse ZBP1.

EMBO reports·2026
Same author

Incorporation of Co-Cyromazine Complexes Onto Halloysite Nanotubes as a Spin-Tip Solid-Phase Extraction Sorbent for Determining Hydrophilic B Vitamins in Functional Foods.

Journal of separation science·2026
Same author

Enhanced Nursing Intervention, Glycemic Variability, And In-Hospital Outcomes In Type 2 Diabetes Mellitus: A Retrospective Cohort Study.

Journal of visualized experiments : JoVE·2026
Same author

HiGATE: hierarchical graph attention for multi-scale tissue encoder in computational pathology.

Frontiers in oncology·2026
Same author

[<i>CNKSR2</i> gene variants causing Houge type of X-linked syndromic intellectual developmental disorder: Seven cases and literature review].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2026
Same author

An ultrasonographic tumor location-based model for predicting central lymph node metastasis in unifocal papillary thyroid microcarcinoma.

Frontiers in endocrinology·2026

Related Experiment Video

Updated: Nov 19, 2025

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

9.8K

Superresolution characterization of core centriole architecture.

Yuan Tian1, Chenxi Wei1, Jianfeng He2

  • 1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.

The Journal of Cell Biology
|February 3, 2021
PubMed
Summary

Researchers mapped the Drosophila centriole core

More Related Videos

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.1K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

2.8K

Related Experiment Videos

Last Updated: Nov 19, 2025

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

9.8K
Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.1K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

2.8K

Area of Science:

  • Cell Biology
  • Structural Biology
  • Biochemistry

Background:

  • The centrosome, a key microtubule-organizing center in animal cells, consists of two centrioles and pericentriolar material.
  • While outer centriole structure is well-studied, the protein architecture of the centriole core remains largely uncharacterized.

Purpose of the Study:

  • To elucidate the protein architecture within the centriole core at high resolution.
  • To understand the spatiotemporal organization of centriolar proteins during centriole biogenesis and its role in centriole-to-centrosome conversion.

Main Methods:

  • Cryo-electron tomography of Drosophila melanogaster centrioles to achieve ~50-nm resolution.
  • Analysis of protein localization and symmetry within the centriole core.

Main Results:

  • A detailed protein map of the Drosophila centriole core was generated.
  • Identified a Sas6 ring overlapping with Cep135 at the C-terminus, with Cep135 exhibiting ninefold symmetry.
  • Discovered Ana3 and Rcd4 with distinct ninefold symmetry, sequentially loaded during biogenesis and crucial for centriole-to-centrosome conversion via the Cep135-Ana1-Asterless complex.

Conclusions:

  • The study provides a high-resolution spatiotemporal map of the centriole core protein architecture.
  • Reveals the sequential recruitment and organization of proteins during centriole biogenesis.
  • Offers insights into the structural mechanisms underlying centriole formation and function.