Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

14.9K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
14.9K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

10.3K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
10.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic and epidemiological analyses of alcohol consumption patterns and age-related macular degeneration risk among current drinkers: a role for TNFRSF10A.

Journal of global health·2026
Same author

Functional targeting of PTTG1 in pericytes restores vascular integrity in diabetic retina.

Science advances·2026
Same author

Active Constituents of <i>Magnolia officinalis</i> in Cancer Management: Molecular Mechanisms and Therapeutic Potential.

The American journal of Chinese medicine·2026
Same author

Amino acid metabolism in retinal diseases: Mechanisms, diagnostics, and therapeutic opportunities.

Experimental eye research·2026
Same author

Obovatol induces apoptosis in breast cancer by downregulating the PI3K/Akt pathway.

Translational cancer research·2026
Same author

Brain-Targeted RVG-Liposomal Melatonin Ameliorates Manganese Neurotoxicity by Enhancing Neurogenesis and Modulating Systemic Amino Acid Profiles.

Journal of pineal research·2026

Related Experiment Video

Updated: Nov 18, 2025

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
06:46

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Published on: May 8, 2017

9.0K

Microarray analysis reveals an inflammatory transcriptomic signature in peripheral blood for sciatica.

Yi Wang1, Guogang Dai2, Ling Jiang3

  • 1Cervicodynia/Omalgia/Lumbago/Sciatica Department 2, Sichuan Provincial Orthopedics Hospital, No. 132 West First Section First Ring Road,Wuhou District, Chengdu, 610041, Sichuan Province, China. yiwang1984@hotmail.com.

BMC Neurology
|February 4, 2021
PubMed
Summary

Sciatica alters peripheral blood gene expression, revealing an inflammatory signature. Key genes involved in immune response were identified, validating changes in sciatica patients.

Keywords:
Differential expressionEnrichmentNetworkPeripheral bloodSciatica

More Related Videos

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

627
Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry
08:47

Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry

Published on: June 24, 2025

404

Related Experiment Videos

Last Updated: Nov 18, 2025

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
06:46

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Published on: May 8, 2017

9.0K
Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

627
Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry
08:47

Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry

Published on: June 24, 2025

404

Area of Science:

  • Peripheral blood transcriptomics
  • Sciatica biomarker discovery
  • Gene expression profiling

Background:

  • Sciatica pathology is well-studied, but peripheral blood transcriptional changes remain uncharacterized.
  • Understanding these changes can reveal diagnostic or prognostic markers for sciatica.

Purpose of the Study:

  • To characterize the peripheral blood transcriptomic signature associated with sciatica.
  • To identify key genes contributing to transcriptional dysregulation in sciatica patients.

Main Methods:

  • Microarray analysis to identify differentially expressed genes (DEGs) in peripheral blood.
  • Functional and network analyses to determine the biological pathways and key genes involved.
  • Quantitative reverse transcription PCR (qRT-PCR) to validate key gene expression levels.

Main Results:

  • 153 genes were differentially expressed in sciatica patients compared to controls (131 upregulated, 22 downregulated).
  • DEGs were significantly enriched in inflammatory and immune response pathways.
  • Key genes including Toll-like receptor 4, matrix metallopeptidase 9, and myeloperoxidase were identified and validated via qRT-PCR.

Conclusions:

  • An inflammatory signature in peripheral blood characterizes sciatica.
  • Specific genes essential for mRNA dysregulation in sciatica were identified, offering potential biomarkers.