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Updated: Nov 18, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Sequencing and characterization of the Megachile strupigera (Hymenoptera: Megachilidae) mitochondrial genome
Dunyuan Huang1,2, Tianjuan Su1, Bo He2
1Key Laboratory of Zoological Systematics and Evolution (CAS), Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Abstract:
The mitochondrial genome (mitogenome) of Megachile strupigera (Hymenoptera: Megachilidae: Megachilinae) was determined in our study. The sequenced region is 15,193 bp with an A + T content of 83.44%, including 13 protein-coding genes, two ribosomal RNAs and 19 transfer RNAs. All PCGs are initiated by typical ATN codons and stop with the complete termination codon TAA, except for nad3 gene, which has an incomplete stop codon T. Bayesian method supported the monophyly of both Megachilidae and Apoidea. And within the Apoidea, Apidae and Megachilidae formed a sister clade to Colletidae.
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