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Related Experiment Video

Updated: Nov 18, 2025

Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies
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E-cigarettes compromise the gut barrier and trigger inflammation.

Aditi Sharma1, Jasper Lee1, Ayden G Fonseca2

  • 1Department of Pathology, University of California, San Diego, CA 92093, USA.

Iscience
|February 4, 2021
PubMed
Summary
This summary is machine-generated.

Chronic exposure to e-cigarette aerosols, even without nicotine, harms the gut barrier. This "non-nicotine" component increases inflammation and disrupts tight junctions, impacting gut health and public health.

Keywords:
Biological SciencesMicrobiologyMicrobiomePhysiologyToxicologyTranscriptomics

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Area of Science:

  • Gastroenterology
  • Toxicology
  • Public Health

Background:

  • E-cigarette use is increasing, raising concerns about the safety of inhaled aerosols.
  • The impact of e-cigarette aerosols on the gut barrier remains incompletely understood.

Purpose of the Study:

  • To investigate the effects of e-cigarette aerosol exposure on the integrity of the gut barrier.
  • To determine whether nicotine-free e-cigarette components contribute to gut barrier dysfunction.

Main Methods:

  • Murine models were used for acute and chronic e-cigarette aerosol inhalation studies.
  • Colon transcriptomics and gut-derived organoid models (murine and human) were employed.
  • Histologic, transcriptome, and co-culture analyses assessed barrier function and inflammation.

Main Results:

  • Chronic, but not acute, exposure to nicotine-free e-cigarette aerosols led to increased gut inflammation.
  • E-cigarette aerosol exposure reduced the expression of tight junction (TJ) markers, compromising barrier integrity.
  • Exposure disrupted barrier function in both murine and human enteroid-derived monolayers (EDMs), even in co-culture with bacteria.

Conclusions:

  • Nicotine-free components of e-cigarettes can negatively impact the gut barrier.
  • E-cigarette use may lead to gut barrier disruption and enhanced inflammation, with implications for chronic diseases.
  • Findings underscore the public health relevance of e-cigarette safety, particularly concerning non-nicotine constituents.