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Genome annotation of disease-causing microorganisms.

Yibo Dong¹, Chang Li¹, Kami Kim²

¹College of Public Health, University of South Florida, Tampa, FL, USA.

Briefings in Bioinformatics

|February 4, 2021

Summary

Genome annotation of pathogenic microorganisms is crucial for biological research. This paper reviews annotation tools and compares their performance across prokaryotic, eukaryotic, and viral genomes.

Keywords:

eukaryote genome annotation pathogenic microorganism prokaryote virus

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Area of Science:

Microbiology
Bioinformatics
Genomics

Background:

Pathogenic microorganisms pose continuous evolutionary challenges to humans.
Advances in genome sequencing yield vast amounts of microbial genomic data.
Genome annotation is essential for interpreting this data but presents significant challenges.

Purpose of the Study:

To summarize genome annotation approaches for pathogenic microorganisms.
To review popular genome annotation tools for prokaryotes, eukaryotes, and viruses.
To provide a comparative analysis of these tools.

Main Methods:

Literature review of genome annotation methodologies.
Selection and categorization of popular genome annotation software.
Comparative performance evaluation using 12 diverse microbial genomes (prokaryotes, eukaryotes, viruses).

Main Results:

Identification of key genome annotation strategies and tools.
Empirical comparison highlighting the strengths and weaknesses of different annotation software.
Discussion of current limitations and future directions in microbial genome annotation.

Conclusions:

Genome annotation tools vary in performance depending on the organism type (prokaryote, eukaryote, virus).
Informed selection of annotation tools is critical for accurate biological interpretation.
Further development is needed to address existing challenges in microbial genome annotation.