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Updated: Nov 18, 2025
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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
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Phage display: an ideal platform for coupling protein to nucleic acid
Huan Qi1, Mingliang Ma1, Danyun Lai1
1Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
Acta Biochimica Et Biophysica Sinica
|February 4, 2021
Summary
Phage display technology, linking surface proteins to DNA, has advanced significantly with next-generation sequencing (NGS). This review covers recent developments and applications in this powerful display technology.
Area of Science:
- Biotechnology
- Molecular Biology
- Genomics
Background:
- Phage display technology relies on the physical linkage of surface proteins to their encoding DNA within a phage particle.
- This technology has broad applications across various scientific and clinical fields.
Purpose of the Study:
- To review and discuss recent advancements in phage display technology.
- To highlight the impact of next-generation sequencing (NGS) and other emerging technologies on phage display.
Main Methods:
- Leveraging phage-displayed peptide/protein/antibody libraries.
- Utilizing next-generation sequencing (NGS) for high-throughput DNA sequencing and decoding.
- Incorporating droplet technology and massive oligonucleotide synthesis.
Main Results:
- High-throughput acquisition of protein-related information is enabled by NGS.
- Recent technological developments have significantly expanded the capabilities and applications of phage display.
- Integration of droplet technology and oligonucleotide synthesis accelerates progress.
Conclusions:
- Phage display technology, enhanced by NGS, offers powerful tools for addressing scientific and clinical questions.
- Continued innovation in display technology promises further breakthroughs in biological research and medicine.
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