Thyroglobulin determination on silane-antibody functionalized interdigitated dielectrode surface to diagnose thyroid...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Oncology

Background:

• Thyroid cancer is a prevalent endocrine malignancy.
• Accurate detection and quantification of thyroglobulin (Tg) are crucial for diagnosis and monitoring.
• Existing diagnostic methods may require improvement in sensitivity and specificity.

Purpose of the Study:

• To develop and validate an electroanalytical sensor for sensitive and specific detection of thyroglobulin.
• To quantify thyroglobulin concentrations using an antibody-functionalized interdigitated electrode (IDE) system.
• To assess the potential of this sensor for thyroid cancer diagnosis.

Main Methods:

• Fabrication of an interdigitated electrode (IDE) surface.
• Immobilization of anti-thyroglobulin antibodies onto the IDE using (3-Aminopropyl)triethoxysilane.
• Electrochemical detection of varying thyroglobulin concentrations.

Main Results:

• The developed IDE sensor demonstrated a dose-dependent response to thyroglobulin.
• Enhanced current responses were observed with increasing thyroglobulin concentrations.
• A low detection limit of 1 pM was achieved with high linearity (R² = 0.9707) and sensitivity.

Conclusions:

• The antibody-functionalized IDE sensor enables specific and sensitive quantification of thyroglobulin.
• Control experiments confirmed the specificity against thyroid peroxidase and non-immune antibodies.
• This electroanalytical approach shows promise for the early diagnosis and monitoring of thyroid cancer.