Related Experiment Video
Updated: Nov 18, 2025
07:37
An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
Published on: June 6, 2025
697
ANGPTL2 Induces Synovial Inflammation via LILRB2
Sayuri Nishiyama1, Naoto Hirose2, Makoto Yanoshita1
1Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Kasumi 1-2-3, Minami-ku, Hiroshima-shi, Hiroshima Prefecture, Japan.
Inflammation
|February 4, 2021
Summary
Angiopoietin-like protein 2 (ANGPTL2) drives inflammation in synovial cells through the LILRB2 receptor. Blocking LILRB2 reduces ANGPTL2-induced inflammatory gene expression and signaling pathways, suggesting LILRB2 as a therapeutic target for osteoarthritis.
Area of Science:
- Biochemistry
- Immunology
- Cell Biology
Background:
- Angiopoietin-like proteins (ANGPTLs) are implicated in tissue repair but ANGPTL2 can promote chronic inflammation.
- ANGPTL2 interacts with receptors like LILRB2, but its role in ANGPTL2-induced inflammation is not fully understood.
Purpose of the Study:
- To investigate the mechanisms of ANGPTL2-induced inflammation in human fibroblast-like synoviocytes (HFLS).
- To elucidate the role of leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) in ANGPTL2-mediated inflammatory signaling.
Main Methods:
- Real-time RT-PCR was used to assess inflammation-related gene expression.
- Western blotting analyzed the phosphorylation of MAPK, NF-κB, and Akt signaling pathways.
- HFLS were treated with ANGPTL2 and an anti-LILRB2 antibody.
Main Results:
- ANGPTL2 significantly increased inflammatory gene expression in HFLS.
- Pretreatment with an anti-LILRB2 antibody attenuated ANGPTL2-induced inflammatory gene expression.
- ANGPTL2 activated ERK, p38, JNK, NF-κB, and Akt phosphorylation, which was inhibited by the anti-LILRB2 antibody.
Conclusions:
- ANGPTL2 induces inflammatory factor expression in synovial cells via LILRB2.
- LILRB2 is a key mediator of ANGPTL2-induced inflammation in HFLS.
- LILRB2 represents a potential therapeutic target for managing matrix degradation in osteoarthritis.
More Related Videos
Related Concept Videos
01:20The JAK-STAT Signaling Pathway
10.3K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
10.3K
01:24T Cell Types and Functions
1.8K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.8K
01:16TGF - β Signaling Pathway
8.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
8.5K
01:28Inflammatory Response
15.0K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
15.0K