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Updated: Nov 18, 2025
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
Detection of Superoxide Anion Oxygen Reduction Reaction Intermediate on Pt(111) by Infrared Reflection Absorption
Valentín Briega-Martos1, William Cheuquepán1, Juan M Feliu1
1Instituto de Electroquímica, Universidad de Alicante, Apdo. 99, E-03080 Alicante,Spain.
Abstract:
In this work, in situ external infrared reflection absorption spectroscopy (IRRAS) is successfully employed for the detection of intermediate species in the oxygen reduction reaction (ORR) mechanism on a flat and well-defined Pt surface. Superoxide anion species (O2-) are detected on the Pt(111) surface in an O2-saturated solution with a NaF/HClO4 mixture with pH 5.5 by the observation of a O-O vibration band at ca. 1080 cm-1. The observation of O2- without the use of any other additional method of signal enhancement is possible because in these experimental conditions O2- is the main ORR-generated intermediate and its reactivity is limited in this pH. This leads to the accumulation of O2- near the Pt surface, facilitating its identification.
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