microRNA-1388-5p inhibits NF-κB signaling pathway in miiuy croaker through targeting IRAK1

Area of Science:

• Immunology
• Molecular Biology
• Genetics

Background:

• The innate immune response is vital for host defense against pathogens.
• MicroRNAs (miRNAs) are key regulators of innate immunity.
• Understanding miRNA functions in fish immunity is crucial for aquaculture and disease resistance.

Purpose of the Study:

• To investigate the role of miR-1388-5p in the innate immune response of miiuy croaker.
• To identify the target gene regulated by miR-1388-5p.
• To elucidate the mechanism by which miR-1388-5p modulates the NF-κB signaling pathway.

Main Methods:

• Bioinformatics analysis to predict miRNA-target interactions.
• Dual-luciferase reporter assays to validate target gene.
• Inhibition of target gene expression using miR-1388-5p mimics and plasmids.
• Assessment of NF-κB pathway activation.

Main Results:

• Bioinformatics predicted IRAK1 as a direct target of miR-1388-5p.
• Dual-luciferase assays confirmed IRAK1 as the target gene.
• miR-1388-5p significantly inhibited IRAK1 expression.
• miR-1388-5p negatively regulated the NF-κB signaling pathway via IRAK1 targeting.

Conclusions:

• miR-1388-5p acts as a negative regulator of the NF-κB pathway in miiuy croaker innate immunity.
• The miR-1388-5p/IRAK1 interaction provides new insights into fish immune regulation.
• This study expands the knowledge of miRNA-mediated regulatory networks in fish.