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Related Experiment Video

Updated: Nov 18, 2025

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

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Recent Advances in Aptamer Sensors.

Samy M Shaban1,2,3, Dong-Hwan Kim1,2

  • 1School of Chemical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea.

Sensors (Basel, Switzerland)
|February 5, 2021
PubMed
Summary
This summary is machine-generated.

Aptasensors, utilizing aptamers for target recognition, offer versatile biosensing capabilities. This review details advances in aptamer-based colorimetric, fluorometric, and electrochemical sensing for diverse applications.

Keywords:
aptamercolorimetric aptasensorelectrochemical aptasensorfluorometric aptasensor

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Aptamers are oligonucleotide-based recognition elements with high affinity for various targets.
  • Antibodies are often difficult to obtain for specific targets like small molecules or metal ions.
  • Aptamers are generated through in vitro selection (SELEX) and offer advantages over antibodies.

Purpose of the Study:

  • To review recent advancements in aptasensors for biosensing applications.
  • To critically discuss the advantages and disadvantages of different aptasensing modalities.
  • To highlight future prospects for aptasensor development.

Main Methods:

  • Review of literature on aptamer selection and aptasensor development.
  • Analysis of aptamer-based colorimetric, fluorometric, and electrochemical sensing strategies.
  • Discussion of applications in environmental monitoring, clinical diagnosis, and drug monitoring.

Main Results:

  • Aptasensors provide high binding affinity and can be engineered for multiple signaling modes.
  • Colorimetric, fluorometric, and electrochemical aptasensing methods offer diverse detection capabilities.
  • Recent advances show promise for aptasensors in various fields, despite some limitations.

Conclusions:

  • Aptasensors represent powerful biosensor probes with broad applicability.
  • Further development is needed to overcome current limitations and expand aptasensor technology.
  • Future prospects include enhanced aptasensor design for diverse and challenging targets.