An Optical Chiral Sensor Based on Weak Measurement for the Real-Time Monitoring of Sucrose Hydrolysis

Area of Science:

• Analytical Chemistry
• Biochemistry
• Physical Chemistry

Background:

• Sucrose hydrolysis is crucial in food and biochemical processes.
• Accurate kinetic studies are essential for understanding reaction mechanisms and optimizing conditions.
• Existing methods may lack real-time monitoring capabilities or require complex sample preparation.

Purpose of the Study:

• To develop a novel chiral sensor for real-time kinetic study of sucrose hydrolysis.
• To utilize weak measurement principles for enhanced optical rotation detection.
• To analyze the influence of enzyme and buffer concentrations on hydrolysis rates.

Main Methods:

• A chiral sensor based on optical rotation detection was designed.
• Weak measurement techniques were employed for sensitive detection of polarization changes.
• Polarization modulation and central wavelength shift analysis were used to determine optical rotation.
• Real-time spectral data acquisition at 100 Hz allowed synchronous fitting and analysis.

Main Results:

• The sensor accurately determined concentration response curves for sucrose, fructose, and glucose.
• Real-time monitoring of sucrose hydrolysis was achieved under varying enzyme and citrate concentrations.
• Distinct hydrolysis velocities were observed and analyzed, demonstrating the method's sensitivity.
• The developed sensor showed high precision in kinetic analysis.

Conclusions:

• The developed chiral sensor with optical rotation detection offers a robust platform for real-time kinetic studies of sucrose hydrolysis.
• Weak measurement provides a sensitive and accurate approach for analyzing chiral compounds.
• This technology holds significant potential for applications in food safety and chemical analysis.