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Updated: Nov 18, 2025
Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis
Published on: October 27, 2023
[Effect of different fluence rates applied in curcumin-photodynamic therapy on Candida albicans biofilms]
Jing Ma1, Ji-Yang Li, Qiao-Zhen Yang
1Department of Stomatology, Huashan Hospital, Fudan University. Shanghai 200040,China.
Purpose:
In this study, the inactivation effect of different fluence rates on Candida albicans biofilms during curcumin-photodynamic therapy was investigated in vitro.
Methods:
The standard Candida albicans and clinical isolated Candida albicans were selected as model fungus and different fluence rates (12, 22, 42, 62, 82, 102 mW/cm2) during curcumin-photodynamic therapy were applied to inactivate Candida albicans biofilm. To evaluate the inactivation effect, XTT assay and Live/Dead kit were employed to quantify and visualize the activities of Candida albicans biofilms. The data were analyzed with SPSS 19.0 software package.
Results:
When 40 μmol/L of curcumin was applied followed by 4 min illumination, both standard Candida albicans and clinical isolated Candida albicans biofilms were greatly inactivated along with the increase of fluence rates. When fluence rate increased to 102 mW/cm2, there was no significant difference between the experimental group and the previous experimental group(P>0.05).
Conclusions:
Fluence rate plays an important role in inactivation of Candida albicans biofilms during curcumin-photodynamic therapy, with optimized value of fluence rate of 82 mW/cm2 in this study.
