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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

14.9K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Updated: Nov 18, 2025

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Flow cytometry: principles, applications and recent advances.

Sonal M Manohar1, Prachi Shah1, Anusree Nair1

  • 1Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS (Deemed-to-be) University, Vile Parle (West), Mumbai 400056, India.

Bioanalysis
|February 5, 2021
PubMed
Summary
This summary is machine-generated.

Flow cytometry (FCM) analyzes cells using light scattering and fluorescence. This review covers its principles, applications, and recent advances in cell analysis technology.

Keywords:
apoptosiscell cycle analysiscell sortingflow cytometerfluorescenceintracellular staining

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Flow cytometry (FCM) is a powerful cell analysis technique.
  • It enables multiparametric analysis of single cells.
  • FCM allows for cell sorting based on specific parameters.

Purpose of the Study:

  • To explain the fundamental principles of FCM.
  • To outline the main applications of FCM in various scientific fields.
  • To review recent technological advancements and innovations in FCM.

Main Methods:

  • Principles of light scattering and fluorescence emission.
  • Single-cell analysis and multiparametric data acquisition.
  • Cell sorting based on physical and fluorescent properties.

Main Results:

  • FCM provides fast, quantitative, and high-resolution cell analysis.
  • It allows for the identification and separation of distinct cell subpopulations.
  • Emerging technologies like imaging FCM and mass cytometry address traditional limitations.

Conclusions:

  • FCM is a versatile and essential tool in biological research.
  • Continuous innovation is expanding the capabilities of FCM.
  • Understanding FCM principles and advances is crucial for researchers.