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Transcript Dynamics in Wounded and Inoculated Scots Pine
Vilnis Šķipars1, Dainis Ruņģis1
1Genetic Resource Centre, Latvian State Forest Research Institute "Silava", 111 Rigas st., LV-2169 Salaspils, Latvia.
International Journal of Molecular Sciences
|February 6, 2021
Summary
Scots pine trees inoculated with Heterobasidion annosum showed significant gene expression changes, with some genes upregulated for stress protection and others downregulated for photosynthesis. This reveals key plant defense pathways against fungal infection.
Area of Science:
- Plant Pathology
- Molecular Biology
- Forest Science
Background:
- Comparative transcriptome analysis is crucial for understanding plant-pathogen interactions.
- Scots pine (Pinus sylvestris) is economically important, and Heterobasidion annosum is a significant pathogen.
Purpose of the Study:
- To investigate gene expression changes in Scots pine ramets following wounding and Heterobasidion annosum inoculation.
- To identify specific genes and pathways involved in the plant's response to fungal infection.
Main Methods:
- Comparative transcriptome analysis of genetically identical Scots pine ramets.
- Analysis of gene expression in control, wounded, and inoculated samples.
Main Results:
- 230 genes upregulated and 116 downregulated in inoculated vs. control samples.
- 32 differentially expressed genes between inoculated and wounded samples (30 upregulated post-inoculation).
- Upregulated genes associated with oxidative stress protection; downregulated genes linked to photosynthesis and drought tolerance.
Conclusions:
- Transcriptome dynamics suggest involvement of auxin, ethylene, jasmonate, gibberellin, and reactive oxygen species pathways.
- Cell wall modification is likely regulated in response to H. annosum infection.
- Results provide insights into Scots pine defense mechanisms against fungal pathogens.
