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Platelet-derived extracellular vesicles express NADPH oxidase-1 (Nox-1), generate superoxide and modulate platelet
Renato Simões Gaspar1, Plinio M Ferreira2, Joanne L Mitchell1
1Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK.
Free Radical Biology & Medicine
|February 6, 2021
Summary
Platelet-derived extracellular vesicles (PDEVs) contain Nox-1, which mediates their ability to activate platelets and generate superoxide. Inhibiting Nox-1 may offer a strategy against thrombotic diseases.
Area of Science:
- Hematology
- Biochemistry
- Cell Biology
Background:
- Platelets release extracellular vesicles (PDEVs) upon activation, a process influenced by reactive oxygen species (ROS).
- Platelet NADPH oxidase-1 (Nox-1) is involved in ROS generation and thrombus formation, acting downstream of the GPVI receptor.
Purpose of the Study:
- To determine if PDEVs contain Nox-1.
- To investigate the role of Nox-1 in PDEV-induced platelet activation.
Main Methods:
- PDEVs were isolated from activated and resting platelets.
- Nanoparticle tracking analysis, flow cytometry, and immunoblot analysis were used to characterize PDEVs.
- ROS production was quantified using DCF fluorescence and electron paramagnetic resonance.
Main Results:
- Nox-1 was present in PDEVs and increased on activated platelet membranes.
- PDEVs induced platelet activation, and this effect was mediated by Nox-1.
- PDEVs generated superoxide, a process partially dependent on Nox-1.
Conclusions:
- PDEVs activate platelets via Nox-1-mediated superoxide generation.
- Nox-1 inhibition abrogated PDEV-mediated platelet activation.
- Targeting Nox-1 presents a potential therapeutic strategy for thrombotic diseases.
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